To probe into the mechanism behind the formation of tetraploids in multiploid induction of pearl oyster, chromosome behaviour in fertilized eggs of Pinctada martensii following inhibition of the first polar body ( PB1) was studied with acetic orcein staining techniques. 为了解在珍珠贝多倍体诱导中四倍体形成的机制,用醋酸地衣红染色技术研究抑制马氏珠母贝(Pinctadamartensii)受精卵第一极体排放后的染色体行为。